Mgr. Markéta Schmidt Černohorská, Ph.D.

Field of specialization: Cellular biology, biophysics, microscopy
Room: CPTO 4.05 (CENAB)
Phone: +420 475 528 6789
E-mail: marketa.schmidt.cernohorska@ujep.cz
OFFICE HOURS: By appointment
ORCID ID: 000-0001-9053-8928
ResearchGATE: https://www.researchgate.net/profile/Marketa-Schmidt-Cernohorska

Education:
2009-2015 Ph.D., Charles University in Prague, Faculty of Science (Developmental and Cellular Biology)
2007-2009 Mgr., Charles University in Prague, Faculty of Science (Genetics, Molecular biology and Virology)
2004-2007 Bc., Palacky University in Olomouc, Faculty of Science (Molecular and Cellular Biology)

Further education:
2025 Short research stay with Brillouin spectroscopy at EMBL, Heidelberg, Germany
2024 Summer School Non-equilibrium processes in Biology and Physics, Erwin Schrödinger Institute, Vienna, Austria
2022-2023 Postdoctoral mentoring program POSTDOC:MUV
2022 EMBO Lab Leadership Course for postdocs (Heidelberg, Germany)
2021-2022 Short research stays with Brillouin spectroscopy at EMBL, Heidelberg, Germany
2022 Short research stays with Atomic force microscopy at TU Vienna, Austria
2022 Short research stays with Optical tweezers microscopy, BIOCEV, Prague, Czechia
2020 Summer School with Brillouin spectroscopy at Ben-Gurion University, Beer Sheva, Israel

Professional research experience:
2025-2028 Global Expert Postdoctoral Fellow at CENAB, Faculty of Sciences, UJEP in Usti and Labem. ((I) Biophysical properties of human centrosomes. (II) Cytoskeleton in Tardigrades. Part of the team in projects: RUR (CZ.10.02.01/00/22_002/0000210), GAČR (grant. num. 25-14392L)
2020-2023 Marie Sklodowska-Curie REWIRE postdoctoral fellow at Max Perutz Labs, University of Vienna, Austria (Biophysical properties of centrosome in C. elegans). Principal investigator: MSCA-REWIRE (grant agr. num. 847693, CentrosomeInsight)
2028-2019 Postdoctoral position at the Department of Adaptive Immunity, Institute of Molecular Genetics, Czech Academy of Science (IMG CAS), Prague, Czechia (BBSome assembly and regulation in ciliogenesis). Part of the team in projects: GACR (grant agr. num. 21-21612S) and ERC Starting (grant agr. num. 802878 FunDiT)
2017-2018 Postdoctoral position at the Department of Cell Biology, Faculty of Science, University of Geneva, Switzerland ((I) Mechanism of microtubule doublet assembly in centriole. (II) Ultra-Expansion microscopy applied to centriole.) Part of the team in project: ERC Starting (grant agr. num. 647403 ISAC)
2016-2017 Research assistant at the Department of Epigenetic Regulations, IMG CAS, Czechia ((I) The role of retrotransposons in gene development and mouse preimplantation development. (II) The role of Dicer in meiotic spindle assembly in mice oocytes.) Part of the team in projects: ERC Consolidator (grant agr. num. 647403, D-FENS) and RVO 68378050
2009-2015 PhD degree study at the Department of Biology of Cytoskeleton, IMG CAS, Czechia. ((I) The role of GIT/PIX-PAK complex in g-tubulin regulation. (II) Functional study of g-tubulin 2 in mouse preimplantation development. (III) Study of the effect of new predesigned anti-cancer drugs on microtubules.) Principal investigator: GAUK (grant agr. num. 888713); part of the team: GACR (grant agr. num. P302/12/1673, 15-22194S, 16-23702S)
2007-2009 Master thesis project at the Department of Genome Integrity, IMG CAS, Czechia. (The role of Jak/STAT signaling pathway in cellular senescence.). Part of the team in project: GACR (grant agr. num. GA204/08/1418), MSMT (grant agr. num. LH12050), MZV (grant agr. num. NT14467), RVO (grant agr. num. RVO 68378050)
2005-2007 Bachelor thesis project, Department of Embryology and Histology, Faculty of Medicine in Hradec Kralové, Charles University in Prague, Czechia. (Morphologic characteristics of endothelial cells and their precursors.)
2002-2004 Student´s Professional Activities (SOČ), Department of Medicine Biology, Faculty of Medicine, Palacký University in Olomouc, Czechia. (Cell cycle in pathogenic yeast Cryptococcus neoformans studied with image analysis.)

Awards and fellowships:
2020-2023 Marie Sklodowska-Curie REWIRE long-term postdoctoral fellowship (264,000 €; University of Vienna)
2022 MOSBRI TNA (MOlecular Scale Biophysics Research Infrastructure – TransNational Access (Institute of Biotechnology CAS, BIOCEV Czech Republic)
2020 COST Action: Brillouin Light Scattering Microspectroscopy for Biological and Biomedical Research and Applications (Ben-Gurion University, Beer Sheva, Israel)
2013-2014 Charles University PhD fellowship (9,600 €, Czech Republic)

Invited talks:
2026 Faculty of Science, Charles Uni in Prague
2025 Erwin Schrödinger Institute, Charged Soft Matter Conference, Vienna, Austria
2024 ISTA, Vienna, Austria
2023 EMBO Practical Course: Super-resolution in light microscopy, IMG CAS, Prague, Czech Republic
2023 Veterinary Medical University, Vienna, Austria
2022 EMBO Practical Course: Super-resolution in light microscopy, IMG CAS, Prague, Czech Republic
2022 Vienna BioCenter International School, lecture series ´How do you do it?´, Vienna, Austria
2019 IMG CAS, Prague, Czech Republic
2019 MPI-CBG, Dresden, Germany
2017 Czech Bioimaging lecture series, IMG CAS, Prague, Czech Republic

Recent major Publications:
Prasai A, Ivashchenko O, Maskova K, Bykova S, Schmidt Cernohorska M, Stepanek O, Huranova M (2024) BBSome-deficient cells activate intraciliary CDC42 to trigger actin-dependent ciliary ectocytosis. EMBO Reports 26(1), doi: 10.1038/s44319-024-00326-z

Gorilak P, Pruzinova M, Vachova H, Olsinova M, Schmidt Cernohorska M, Varga V (2021) Expansion microscopy facilitates quantitative super-resolution studies of cytoskeletal structures in kinetoplastid parasites. Open Biol, 11(9): 210131. DOI: 10.1098/rsob.210131

Prasai A, Schmidt Cernohorska M, Ruppova K, Niederlova V, Andelova M, Draber P, Stepanek O, Huranova M (2020) The BBSome assembly is spatially controlled by BBS1 and BBS4 in human cells. J Biol Chem 295(42): p. 14279-14290, DOI: 10.1074/jbc.RA120.013905

Dobbelaere J, Schmidt Cernohorska M, Huranova M, Slade D, Dammermann A (2020) Cep97 Is Required for Centriole Structural Integrity and Cilia Formation in Drosophila. Curr Biol, 30(15): p3045-3056, DOI: 10.1016/j.cub.2020.05.078.

Schmidt-Cernohorska M, Zhernov I, Le Guennec M, Achek R, Demurtas D, Mouawad L, Lansky Z, Hamel V, Guichard P (2019) Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails. Science, Jan 18, 363(6424): p. 285-288, DOI: 10.1126/science.aav2567

Gambarotto D, Zwettler FU, Schmidt-Cernohorska M, Fortun D, Borgers S, Heine J, Schoetel JG, Reuss M, Unser M, Boyden ES, Sauer M, Hamel V, Guichard P. (2018) Imaging cellular ultrastructures using expansion
microscopy (U-ExM). Nature Methods, Jan 16(1): p. 71-74, DOI: 10.1038/s41592-018-0238-1

Other Scientific activities:
Elected Scientific Ambassador for EuroBioImaging 2026